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KD-Validated Anti-Ferritin heavy chain 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

     
  • WB - KD-Validated Anti-Ferritin heavy chain 1 Rabbit Monoclonal Antibody AGI2339
    Western blotting analysis using anti-Ferritin heavy chain 1 antibody (Cat#68721). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ferritin heavy chain 1 antibody (Cat#68721, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • WB - KD-Validated Anti-Ferritin heavy chain 1 Rabbit Monoclonal Antibody AGI2339
    Western blotting analysis using anti-Ferritin heavy chain 1 antibody (Cat#68721).Ferritin heavy chain 1 expression in wild type (WT) and ferritin heavy chain 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates.β-Tubulin serves as a loading control. The blot was incubated with anti-Ferritin heavy chain 1 antibody (Cat#68721, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
    detail
  • FC - KD-Validated Anti-Ferritin heavy chain 1 Rabbit Monoclonal Antibody AGI2339
    Flow cytometric analysis of Ferritin heavy chain 1 expression in HepG2 cells using Ferritin heavy chain 1 antibody (Cat#68721, 1:2,000). Green, isotype control; red, Ferritin heavy chain 1.
    detail
  • ICC - KD-Validated Anti-Ferritin heavy chain 1 Rabbit Monoclonal Antibody AGI2339
    Immunocytochemical staining of HepG2 cells with Ferritin heavy chain 1 antibody (Cat#68721, 1:1,000). Nuclei were stained blue with DAPI; Ferritin heavy chain 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, FC, ICC
Primary Accession P02794
Reactivity Rat, Human, Mouse
Clonality Monoclonal
Isotype Rabbit IgG
Clone Names 23GB 1835
Calculated MW Predicted, 21 kDa; Observed, 15 kDa
Gene Name FTH1
Aliases FTH1; Ferritin Heavy Chain 1; FTH; PIG15; FTHL6; PLIF; FHC; Cell Proliferation-Inducing Gene 15 Protein; Proliferation-Inducing Protein 15; Placenta Immunoregulatory Factor; Ferritin, Heavy; Polypeptide 1; Ferritin Heavy Chain; Ferritin H Subunit; Apoferritin; EC 1.16.3.1; H-Ferritin; HFE5
Immunogen A synthesized peptide derived from human Ferritin
Additional Information
Gene ID 2495
Other Names Ferritin heavy chain, Ferritin H subunit, 1.16.3.1, Cell proliferation-inducing gene 15 protein, Ferritin heavy chain, N-terminally processed, FTH1, FTH, FTHL6
Protein Information
Name FTH1
Synonyms FTH, FTHL6
Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity (PubMed:9003196). Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation (PubMed:9003196). Also plays a role in delivery of iron to cells (By similarity). Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes is mediated by the cargo receptor NCOA4 for autophagic degradation and release of iron (PubMed:24695223, PubMed:26436293).
Cellular Location Cytoplasm. Lysosome. Cytoplasmic vesicle, autophagosome
Tissue Location Expressed in the liver.
Research Areas
Citations (0)
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$ 149.00
$ 499.00
Cat# AGI2339
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